Measurement of Poly(ADP-ribose) Level with Enhanced Slot Blot Assay with Crosslinking
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چکیده
منابع مشابه
construction of vector fields with positive lyapunov exponents
in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
15 صفحه اولQuantitative immuno-slot blot assay to measure ACMPK protein in cell extracts of Aspergillus nidulans.
A primary means of detecting and identifying specific proteins in cellular extracts is by Western blot analysis (4,13). Quantitation of a protein of interest is generally accomplished using an enzyme-linked immunosorbent assay (ELISA; References 8, 9 and 11) or radioimmunoassay (4,8). When ELISA or radioimmunoassay procedures for the protein of interest are unavailable, densitometric analysis o...
متن کاملSpecific detection of native multi-subunit proteins by slot-blot assay.
Detection of membrane-bound proteins by immunoblot (Western blot) is by far the most commonly used procedure for detection or quantitation of an antigen (11). However, since this technique requires analysis of the antigen on a denaturing gel using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the antigen generally loses its native conformation (7). Although resolution ca...
متن کاملImmuno slot-blot assay using a membrane which covalently binds protein.
The use of a recently available commercial membrane (Immunodyne Immunoaffinity), which covalently binds proteins, in the development of a quantitative immunological slot blot assay is demonstrated. This membrane was found to be valuable in the quantification of polygalacturonase from tomato fruit, a protein which binds weakly to nitrocellulose membrane. The covalent binding of protein to a soli...
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ژورنال
عنوان ژورنال: Challenges
سال: 2018
ISSN: 2078-1547
DOI: 10.3390/challe9020027